How to Grow Psilocybin Mushrooms at Home The FOOLPROOF Way: An All-Inclusive Beginner's Guide to Easily Grow Psychedelic Magic Mushrooms in Your Own Home (The Complete Guide to Psilocybin Mushrooms) by Alan Alpert

Author:Alan Alpert [Alpert, Alan]
Language: eng
Format: epub
Published: 2022-03-06T23:00:00+00:00

Chapter 6 – Making A Spore Print, Spore Syringe, Vial, and Liquid Culture

Any grower who wants to grow crop after crop of mushrooms is either going to have to buy a new spore syringe for every crop or better, make their own spore print, spore syringe, spore vial, and liquid culture so they can inoculate crops indefinitely. Making a spore print is a fairly straightforward technique that requires sterility. In order to properly make a spore print, we are going to need a laboratory glove box or sterile box. If neither are available, the open oven technique is also a distant option. The sterile technique chosen will depend on budget, supplies, and motivation. Additionally, we will need to make an alcohol lamp in order to flame sterilize the instruments we will use. We can re-use the original syringe that the spores were shipped in by sterilizing it in the pressure cooker or fill a new sterile syringe.

Creating A Sterile Environment

In order of most ornate to least, we will discuss the laboratory glove box, the sterile box, and the open oven technique. The laboratory glove box is the best option available, yet not available to most home growers. A sterile box is the option that most growers choose, as it isn’t hard to make on a budget and offers a sterile space with limited potential contamination. The open oven technique capitalizes on the principle that hot air rises in order to push any contaminants far away from the spore prints, spore syringe, and liquid culture. While not optimal, sterile techniques performed with the open oven technique can be done in a pinch and doesn’t require any extra supplies.

Negative Air Chamber - Laboratory Glove Box

Growers who have access to a laboratory glove box should use it for all sterile procedures, as this is the most sterile option available. Equipped with a HEPA filter and vacuum suction that pulls in inert gas like nitrogen without oxygen, nothing beats this professional option. However, most home growers will not have access to a laboratory glove box so the sterile box option is the next best substitute.

Sterile Box

A sterile box requires a plastic bin with a cover and a pair of heavy duty rubber gloves. In order to make the sterile box, drill out two holes in one of the long sides of the plastic bin big enough to fit your hands. The holes should be a comfortable distance apart so that both hands can be inserted into the sterile box comfortably. The best way to drill holes into the plastic bin is to use a circular drill bit, drilling from the inside out, while holding a wooden block on the other side so the plastic doesn’t crack. As with the monotub technique, allow the drill bit to do all the work and avoid putting too much pressure on the plastic bin.

After the holes are drilled out, sand down any rough edges and tape the open end of a pair of heavy duty rubber gloves into the holes.

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